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T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate.","species":" postnatal day 6 (P6±1d) FVB mice","animal_model":"apical turn outer hair cells ( low-frequency processing  OHCs) from intact cochlear (organ of Corti) explants","criteria":"","result":false},{"rec_id":"RC03984","func":"F021214","targ":null,"compound":"D1411: Gentamicin","paper":"312","func_act":"decrease","targ_act":"","dose":"300 μg/ml","time":"1hr","method":"scanning confocal microscope (NADH autofluorescence); T1 imaging buffer contained 5 mM glucose. T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate.","species":" postnatal day 6 (P6±1d) FVB mice","animal_model":"apical turn inner hair cells ( low-frequency processing  IHCs) from intact cochlear (organ of Corti) explants","criteria":"","result":false},{"rec_id":"RC03985","func":"F021214","targ":null,"compound":"D1411: Gentamicin","paper":"312","func_act":"decrease","targ_act":"","dose":"300 μg/ml","time":"1hr","method":"scanning confocal microscope (NADH autofluorescence); T1 imaging buffer contained 5 mM glucose. T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate.","species":" postnatal day 6 (P6±1d) FVB mice","animal_model":"basal turn outer hair cells ( low-frequency processing  OHCs) from intact cochlear (organ of Corti) explants","criteria":"","result":true},{"rec_id":"RC03986","func":"F021214","targ":null,"compound":"D1411: Gentamicin","paper":"312","func_act":"decrease","targ_act":"","dose":"300 μg/ml","time":"1hr","method":"scanning confocal microscope (autofluorescence); T1 imaging buffer contained 5 mM glucose. T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate.","species":" postnatal day 6 (P6±1d) FVB mice","animal_model":"basal turn inner hair cells ( high-frequency processing  IHCs) from intact cochlear (organ of Corti) explants","criteria":"","result":false},{"rec_id":"RC03987","func":null,"targ":"T002","compound":"D1411: Gentamicin","paper":"312","func_act":"","targ_act":"inhibit","dose":"300 μg/ml","time":"1hr","method":" Succinate dehydrogenase (SDH) histochemistry (tetranitro-blue accumulation)","species":" postnatal day 6 (P6±1d) FVB mice","animal_model":"apical turn ( low-frequency processing)  outer hair cells (OHCs) from intact cochlear (organ of Corti) explants","criteria":"","result":true},{"rec_id":"RC03988","func":null,"targ":"T002","compound":"D1411: Gentamicin","paper":"312","func_act":"","targ_act":"inhibit","dose":"300 μg/ml","time":"1hr","method":" Succinate dehydrogenase (SDH) histochemistry (tetranitro-blue accumulation)","species":" postnatal day 6 (P6±1d) FVB mice","animal_model":"apical turn ( low-frequency processing)  outer hair cells (OHCs) from intact cochlear (organ of Corti) explants","criteria":"","result":false},{"rec_id":"RC03989","func":null,"targ":"T002","compound":"D1411: Gentamicin","paper":"312","func_act":"","targ_act":"inhibit","dose":"300 μg/ml","time":"1hr","method":" Succinate dehydrogenase (SDH) histochemistry (tetranitro-blue accumulation)","species":" postnatal day 6 (P6±1d) FVB mice","animal_model":"basal turn(high-frequency regions of the cochlea) outer hair cells  from intact cochlear (organ of Corti) explants","criteria":"","result":true},{"rec_id":"RC03990","func":null,"targ":"T002","compound":"D1411: Gentamicin","paper":"312","func_act":"","targ_act":"inhibit","dose":"300 μg/ml","time":"1hr","method":" Succinate dehydrogenase (SDH) histochemistry (tetranitro-blue accumulation)","species":" postnatal day 6 (P6±1d) FVB mice","animal_model":"basal turn(high-frequency regions of the cochlea) outer hair cells from intact cochlear (organ of Corti) explants","criteria":"","result":false},{"rec_id":"RC03991","func":"F0504","targ":null,"compound":"D1404: Staurosporine","paper":"313","func_act":"increase","targ_act":"","dose":"2 µM","time":"24hr","method":"mt-roGFP and mitoSOX","species":"","animal_model":" U2OS","criteria":"","result":true},{"rec_id":"RC03992","func":"F0504","targ":null,"compound":"D1448: camptothecin","paper":"313","func_act":"increase","targ_act":"","dose":"30 µM","time":"24hr","method":"mt-roGFP and mitoSOX","species":"","animal_model":" U2OS","criteria":"","result":true},{"rec_id":"RC03993","func":"F0504","targ":null,"compound":"D0194: Menadione","paper":"313","func_act":"increase","targ_act":"","dose":"20 µM","time":"24hr","method":"mt-roGFP and mitoSOX","species":"","animal_model":" U2OS","criteria":"","result":true},{"rec_id":"RC03994","func":"F0504","targ":null,"compound":"D0029: Cisplatin","paper":"313","func_act":"increase","targ_act":"","dose":"150 µM","time":"24hr","method":"U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria","species":"","animal_model":" U2OS","criteria":"","result":true},{"rec_id":"RC03995","func":"F0504","targ":null,"compound":"D1448: camptothecin","paper":"313","func_act":"increase","targ_act":"","dose":"30 µM","time":"24hr","method":"U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria","species":"","animal_model":" U2OS","criteria":"","result":true},{"rec_id":"RC03996","func":"F0104","targ":null,"compound":"D0029: Cisplatin","paper":"313","func_act":"induce","targ_act":"","dose":"","time":"","method":"release of Smac m cherry(IMS-RP probe is a non-functional variant of mitochondrial protein Smac - mCherry fusion protein that acts as an indicator of mitochondrial permeabilization in live cells)","species":"","animal_model":" U2OS","criteria":"","result":true},{"rec_id":"RC03997","func":"F0504","targ":null,"compound":"D0371: Mitoquinone","paper":"313","func_act":"reduce","targ_act":"","dose":"","time":"","method":"mt-roGFP ratio change (Quantitative representation of the percentage of cell death in cells pre-treated with MitoQ followed by drug treatment or drug alone as indicated for 24 h)","species":"","animal_model":" U2OS","criteria":"","result":true},{"rec_id":"RC03998","func":"F060301","targ":"T231","compound":"D1447: LDC203974","paper":"314","func_act":"inhibit","targ_act":"inhibit","dose":" 0–10 μM","time":"","method":"Single nucleotide incorporation assays at varying ATP concentrations","species":"","animal_model":"","criteria":"Vmax app, maximum reaction rate; Km app, substrate affinity","result":true},{"rec_id":"RC03999","func":"F060301","targ":"T231","compound":"D1447: LDC203974","paper":"314","func_act":"inhibit","targ_act":"inhibit","dose":"743nM","time":"","method":"Microscale thermophoresis binding curves in the absence or presence of 10 μM IMT1B ","species":"","animal_model":"","criteria":"Kd","result":true},{"rec_id":"RC04000","func":"F060301","targ":"T231","compound":"D1447: LDC203974","paper":"314","func_act":"inhibit","targ_act":"inhibit","dose":"10 μM","time":"6hr","method":" Mitochondrial transcript levels of ND1 and CYTB","species":"","animal_model":"HeLa cells","criteria":"","result":true},{"rec_id":"RC04001","func":"F01","targ":null,"compound":"D1411: Gentamicin","paper":"316","func_act":"reduce","targ_act":"","dose":" 2.5 mM","time":"","method":"MtMP was investigated with the use of safranin, a biological stain with an excitation wavelength of 495 nm and an emission wavelength of 587 nm. ","species":"rat","animal_model":"isolated rat liver (and kidney) mitochondria","criteria":"","result":true},{"rec_id":"RC04002","func":"F01","targ":null,"compound":"D1411: Gentamicin","paper":"316","func_act":"reduce","targ_act":"","dose":"","time":"","method":"Rhodamine-123","species":"mouse","animal_model":"Mouse Cochlear Culture Sensory Hair Cells","criteria":"","result":true},{"rec_id":"RC04003","func":"F050401","targ":null,"compound":"D1411: Gentamicin","paper":"316","func_act":"reduce","targ_act":"","dose":"5mM","time":"10min","method":"Mitochondrial ROS production can be measured using Amplex Red, a dye that fluoresces dependent on hydrogen peroxide (H2O2) levels. ","species":"rat","animal_model":"isolated rat liver (and kidney) mitochondria","criteria":"","result":true},{"rec_id":"RC04004","func":"F02010102","targ":null,"compound":"D1411: Gentamicin","paper":"316","func_act":"increase","targ_act":"","dose":"5mM","time":"10min","method":"Oroboros O2K Oxygraph, complex II-based state 4 respiration ","species":"rat","animal_model":"isolated rat liver (and kidney) mitochondria","criteria":"","result":true},{"rec_id":"RC04005","func":"F02010106","targ":null,"compound":"D1411: Gentamicin","paper":"316","func_act":"decrease","targ_act":"","dose":"5mM","time":"10min","method":"Oroboros O2K Oxygraph, complex II-based state 3u respiration ","species":"rat","animal_model":"isolated rat liver (and kidney) mitochondria","criteria":"","result":true}]}