F02010202 | Oxygen consumption rate (OCR)
Function of mitochondria >> Respiration >> Mitochondrial parameters >> oxygen consumption rate (OCR)
| Compound | Dose | Time | Species | Model | Method | Action | Result | Positive criterion | Reference |
|---|---|---|---|---|---|---|---|---|---|
| Entacapone | 33 μM | 2 minutes | human | HepG2 | Measurement of OCR | increase | Positive | EC50 | 7 |
| Nilutamide | 66 μM | 2 minutes | human | HepG2 | Measurement of OCR | decrease | Positive | EC50 | 7 |
| Nimesulide | 1 mM | rat; Sprague-Dawley Crl:CD (SD) BR | lung microsomes | measurement of oxygen consumption | increase | Positive | p < 0.05 | 11 | |
| Flutamide | > 100 μM | 2 minutes | human | HepG2 | Measurement of OCR | decrease | Positive | EC50 | 7 |
| Chlorpromazine | > 300 μM | 2 minutes | human | HepG2 | Measurement of OCR | increase | Positive | EC50 | 7 |
| Diflunisal | 15 μM | 2 minutes | human | HepG2 | Measurement of OCR | increase | Positive | EC50 | 7 |
| Benoxaprofen | 30 μM | 2 minutes | human | HepG2 | Measurement of ECAR | decrease | Positive | EC50 | 7 |
| Troglitazone | > 100 μM | 2 minutes | human | HepG2 | Measurement of ECAR | decrease | Positive | EC50 | 7 |
| Ciglitazone | > 100 μM | 2 minutes | human | HepG2 | Measurement of ECAR | decrease | Positive | EC50 | 7 |
| Troglitazone | 10 μM | 2 minutes | feline | cardiomyocytes | Measurement of OCR | increase | Positive | EC50 | 7 |
| Ciglitazone | > 100 μM | 2 minutes | feline | cardiomyocytes | Measurement of OCR | decrease | Positive | EC50 | 7 |
| Rosiglitazone | > 100 μM | 2 minutes | feline | cardiomyocytes | Measurement of OCR | increase | Positive | EC50 | 7 |
| Yohimbine | mouse | neuron | Oxygen consumption rate (OCR) or extracellular acidification rate (ECAR) measurements from permeabilized neurons and isolated brain mitochondria were performed using the XF96 Extracellular Flux analyzer (Seahorse Bioscience). | increase | Positive | 269 | |||
| Dyclonine | mouse | neuron | Oxygen consumption rate (OCR) or extracellular acidification rate (ECAR) measurements from permeabilized neurons and isolated brain mitochondria were performed using the XF96 Extracellular Flux analyzer (Seahorse Bioscience). | increase | Positive | 269 | |||
| Berberine | 1-10 µM | isolated rat muscle mitochondria | Clark type oxygen electrode (Strathkelvin Instruments) | decrease | Positive | 328 | |||
| ionomycin | rat | liver mitochondria | Oxygen consumption and medium pH were monitored simultaneously with a dual channel Gilson oxygraph equipped with a Clark electrode (Yellow Springs Instruments) and a combination pH electrode (Beckman No. 39030). | increase | Positive | 202 | |||
| buformin | 25 μM | 24 hours | human | HepG2 cells | Measurement of OCR | decrease | Positive | p < 0.001 | 15 |
| Phenformin | 25 μM | 24 hours | human | HepG2 cells | Measurement of OCR | decrease | Positive | p < 0.001 | 15 |
| Rotenone | 263±43 nM | 2 minutes | human | HepG2 | Measurement of OCR | decrease | Positive | EC50 | 7 |
| Antimycin A | 4±0.2 nM | 2 minutes | human | HepG2 | Measurement of OCR | decrease | Positive | EC50 | 7 |
| Oligomycin | 0.92± 0.18 μM | 2 minutes | human | HepG2 | Measurement of OCR | decrease | Positive | EC50 | 7 |
| FCCP | 54±7 nM | 2 minutes | human | HepG2 | Measurement of OCR | increase | Positive | EC50 | 7 |
| 2-Deoxy-D-glucose | 150 mM | 2 minutes | human | HepG2 | Measurement of OCR | increase | Positive | EC50 | 7 |
| Tolcapone | 3.7 μM | 2 minutes | human | HepG2 | Measurement of OCR | increase | Positive | EC50 | 7 |