Compound	Dose	Time	Species	Model	Method	Result	Positive criterion	Reference
Otivarin	0.2 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	98
phenoxan	0.04 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	94
Thiangazole	0.04 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	94
myxalamid pi	15 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	94
phenalamid a2	0.6 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	94
Aurachin a	8.0 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	94
Myxothiazol	20 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	99
stigmatellin	100 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	99
TDS	40 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	99
aureothin	0.3 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	94
Cochlioquinone b	83 nmol/mg		bovine	mitochondria	NADH:O2	Positive	IC50	100
strobilurin a	>100 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	99
pterulone	36 μM		bovine	mitochondria	NADH:O2	Positive	IC50	101
Capsaicin	20–30 μM		bovine	mitochondria	NADH–Q	Positive	IC50	102
Capsaicin	20–30 μM		bovine	mitochondria	NADH:O2	Positive	IC50	102
Rhein	30 μM		bovine	mitochondria	NADH:O2	Positive	IC50	104
Papaverine	5 μM		bovine	mitochondria	NADH–Q-1	Positive	IC50	30
Ubiquinone-2	2 μM		bovine	mitochondria	NADH–Q-1	Positive	IC50	106
Ubiquinone-3	40 μM		bovine	mitochondria	NADH–Q	Positive	IC50	107
Idebenone	0.4 μM				NADH–Q	Positive	IC50	106
Pyridaben	2.4 nM				NADH–Q-1	Positive	IC50	108
Fenpyroximate	4.6 nM				NADH–Q-1	Positive	IC50	108
Fenpyroximate	18 nM				NADH–Q	Positive	IC50	103
Tebufenpyrad	6 nM				NADH–Q-1	Positive	IC50	108
fenazaquin	20 nM				NADH–Q-1	Positive	IC50	109
Benzimidazole	3 nM				NADH–Q	Positive	IC50	94
cyhalothrin	0.6 μM				NADH–DQ	Positive	IC50	110
6-chloro-benzothiadiazole	0.1 mM				NADH:O2	Positive	IC50	111
2M-TIO	40 μM				NADH–Q-1	Positive	IC50	112
amytal	0.2 mM				NADH–Q-1	Positive	IC50	112
meperidine	0.1 mM				NADH–Q-1	Positive	IC50	108
MPP+	0.3 mM				NADH:O2	Positive	IC50	114
MPP+	2–4 mM				NADH–Q	Positive	IC50	115
4'-decyl-mpp+	1.7 μM				NADH:O2	Positive	IC50	115
MQ18	0.2 μM				NADH–Q-1	Positive	IC50	116
2-methylharmine	0.18 mM				NADH:O2	Positive	IC50	117
TIQ	2 mM				NADH:O2	Positive	IC50	118
Haloperidol	3 μM				NADH–Q-1	Positive	IC50	119
HPP+	15 μM				NADH–Q-1	Positive	IC50	119
Dequalinium Chloride	11 μM				NADH:O2	Positive	IC50	120
Cinnarizine	5–10 μM				NADH–Q-1	Positive	IC50	121
para-nonylphenol	20 μM				NADH–Q	Positive	IC50	108
Catechol	0.4 mM				NADH–Q	Positive	IC50	108
CCCP	85 μM				NADH–Q-1	Positive	IC50	108
Erythrosin 5'-iodoacetamide	20 nM				NADH–O2	Positive	IC50	126
Safranine	17 μM				NADH–Q	Positive	IC50	127
dioc5(3)	1.5 μM				NADH–Q	Positive	IC50	127
dioc6(3)	80 nM				NADH–O2	Positive	IC50	126
Diphenyleneiodonium chloride	0.23 μM				NADH–Q-1c	Positive	IC50	128
N,N'-dicyclohexylcarbodiimide	0.1 mM				NADH–Q-1	Positive	IC50	129
o-phenanthroline	0.25 mM				NADH–Q	Positive	IC50	130
amytal						Positive		185
Pioglitazone						Positive		22
Rotenone						Positive		185
Deguelin						Positive		185
Berberine	1-10 µM			isolated rat muscle mitochondria	Clark type oxygen electrode (Strathkelvin Instruments)	Positive		328
Rotenone						Positive		185
Chlorpromazine			rat	isolated liver mitochondria		Positive		23
Haloperidol			rat	isolated liver mitochondria		Positive		23
Risperidone			rat	isolated liver mitochondria		Positive		23
quetiapine			rat	isolated liver mitochondria		Positive		23
Clozapine			rat	isolated liver mitochondria		Negative		23
olanzapine			rat	isolated liver mitochondria		Negative		23
Thioridazine			rat	isolated liver mitochondria		Negative		23
sertraline						Positive		307
Fluoxetine						Positive		307
MPTP						Positive		185
Haloperidol						Positive		185
Papaverine						Positive		30
Acetaminophen						Positive		35
Acetylsalicylic acid						Positive		35
Flutamide						Positive		35
Ketoconazole						Positive		35
Metformin						Positive		35
Nefazodone						Positive		35
Nitrofurantoin						Positive		35
Paraquat						Positive		35
paroxetine						Positive		35
Phenformin						Positive		35
Pioglitazone						Positive		35
Rosiglitazone						Positive		35
Rotenone						Positive		35
Amiodarone						Positive		36
Chlorpromazine						Positive		7
Diclofenac						Positive		39
Flutamide						Positive		41
Ketoconazole						Positive		36
lovastatin						Positive		36
Rosiglitazone						Positive		7
Simvastatin						Positive		7
imipramine						Positive		36
sertraline						Positive		48
zoniporide						Positive		50
Ciprofloxacin						Positive		307
Gemfibrozil				isolated mitochondria		Positive		7
citalopram			pig	brain mitochondria		Positive		62
Berberine	7			LUHMES (Lund human mesencephalic) cells	Glc–Gal–NeuriTox assay	Positive	EC25(NA) [Glc/Gal]	326
Haloperidol						Positive		23
Paraquat	10 mg/kg/week intraperitoneal (i.p.)	4 weeks	C57BL/6 mice			Positive		242
Deguelin	450			LUHMES (Lund human mesencephalic) cells	Glc–Gal–NeuriTox assay	Positive	EC25(NA) [Glc/Gal]	326
Rotenone	10–100 nmol			SK-N-MC Neuroblastoma cells		Positive		242
Fenpyroximate	5600			LUHMES (Lund human mesencephalic) cells	Glc–Gal–NeuriTox assay	Positive	EC25(NA) [Glc/Gal]	326
MPP	30			LUHMES (Lund human mesencephalic) cells	Glc–Gal–NeuriTox assay	Positive	EC25(NA) [Glc/Gal]	326
Rotenone	51			LUHMES (Lund human mesencephalic) cells	Glc–Gal–NeuriTox assay	Positive	EC25(NA) [Glc/Gal]	326
Tebufenpyrad	2700			LUHMES (Lund human mesencephalic) cells	Glc–Gal–NeuriTox assay	Positive	EC25(NA) [Glc/Gal]	326
Piericidin A						Positive		53
rolliniastatin-2						Positive		53
Idebenone						Positive		53
Rotenone						Positive		53
piericidin a						Positive		53
piericidin b						Positive		53
aureothin						Positive		53
amytal						Positive		53
4-alkyl-acridones						Positive		53
4′-alkyl-mpp+ analoguesc						Positive		53
phenoxan						Positive		53
quinol products						Positive		53
reduced q-2b						Positive		53
Myxothiazol						Positive		53
stigmatellin						Positive		53
TDS						Positive		53
2M-TIO						Positive		53
meperidine						Positive		53
para-nonylphenol						Positive		53
Diphenyleneiodonium chloride						Positive		69
ethoxyformic anhydride						Positive		70
Polymyxin B	1.6 μg/ml		Mycobacterium smegmatis	membrane-bound dehydrogenases		Positive	IC50	76
nanaomycin a	31 μg/ml		Mycobacterium smegmatis	membrane-bound dehydrogenases		Positive	IC50	76
Acetaminophen	348.5 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Acetylsalicylic acid	> 800 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
alpidem	25.6 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Amantadine	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Ambroxol	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Amiodarone	45.92 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
british anti-lewisite						Positive		80
Ampicillin	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Antipyrine	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Arsenic trioxide	< 50 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Biotin	44.5 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
lumiracoxib	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
bisacodyl	50.9 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
estradiol acetate	> 200 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Bupivacaine	60.6 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Busulfan	169.8 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Butein	29.6 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Caffeine	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Capsaicin	15 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Carbamazepine	53.4 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Cefixime	41.8 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Chlorambucil	138.7 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Clodronate	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Clotrimazole	2.9 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
curcumin	> 200 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Daunorubicin	12.8 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
dexamethasone valerate	> 200 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Diazoxide	4.9 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Diclofenac	9.1 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Diflunisal	9.8 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Doxorubicin	15.9 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
erlotinib	328.8 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Fluconazole	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Flufenamic Acid	1.7 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Flutamide	ND	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Negative	EC20	36
gefitinib	269.6 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
genistein	81.3 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Glimepiride	16.6 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Gossypol	30.3 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Ibuprofen	170.1 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Imatinib	ND	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Negative	EC20	36
Indomethacin	25.2 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Isoniazid	59.8 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Ketoconazole	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Lamivudine	160.7 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Lidocaine	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Lonidamine	18.9 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
lovastatin	4.4 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
lumiracoxib	26.3 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Manganese	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Mefenamic acid	10.1 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Metformin	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Mitomycin	4.9 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Mitoxantrone	ND	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Negative	EC20	36
Nicotine	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
nifuroxazide	61.5 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Nimesulide	< 25 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Nitrofurantoin	232.3 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Perhexiline	88.4 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Piroxicam	224.5 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
pravastatin	5 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
propylparaben	63 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Pyrazinamide	107.5 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
resveratrol	7.7 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
riboflavin	264.6 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
rifampicin	124.1 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
ritonavir	35.5 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Salicylic acid	354.4 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Simvastatin	1.6 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
sorafenib	283.4 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Sulindac	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Tamoxifen	ND	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Negative	EC20	36
Tolcapone	ND	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Negative	EC20	36
Amiodarone	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Positive	p < 0.05	3
Tolfenamic Acid	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Troglitazone	3.9 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Valproic Acid	> 400 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Zidovudine	> 800 µM	60 mins	mouse	liver mitochondria	Oxygen consumption was monitored with 50nM MitoXpress ( an oxygen-sensitive phosphorescent dye) using a spectrofluorimeter (Tecan Infinite 200; λExcitation 380nm; λEmission 650nm). Rotenone (2µM) was used as 100% baseline for complex I inhibition.	Positive	EC20	36
Benoxaprofen	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Bezafibrate	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Chlorpromazine	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Dantrolene	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Diclofenac	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Fluconazole	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
gefitinib	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Linezolid	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Metformin	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Nefazodone	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Positive	p < 0.001	3
paroxetine	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Positive	p < 0.01	3
pravastatin	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Primaquine	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
radicicol	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Rosiglitazone	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Simvastatin	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Positive	p < 0.01	3
Tamoxifen	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
trazodone	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Trovafloxacin	50 μM		bovine	heart mitochondria	Measurement of complex I activity	Negative	p < 0.05	3
Tamoxifen	15 μM		bovine	heart mitochondria	Measurement of complex II + III activity	Positive	IC50	3
Peroxynitrite	2mM, IC50=1.52mM		rat	heart mitochondria	rate of NADH-dependent ferrycynide reduction at 420nm	Positive	IC50	298
Metformin	66 mM		Bovine	heart mitochondria	Measurement of complex I activity	Positive	IC50	15
Tamoxifen	4 μM		bovine	heart mitochondria	Measurement of complex I activity	Positive	IC50	3
buformin	11.3 mM		Bovine	heart mitochondria	Measurement of complex I activity	Positive	IC50	15
Phenformin	1.2 mM		Bovine	heart mitochondria	Measurement of complex I activity	Positive	IC50	15
Rotenone	0.07 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	94
Rotenone	0.04 nmol/mg		bovine	mitochondria	NADH–O2	Positive	IC50	94
Deguelin	4.2 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	95
Piericidin A	0.06 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	94
Piericidin A	0.03 nmol/mg		bovine	mitochondria	NADH:O2	Positive	IC50	94
Ubicidin-3	0.17 nmol/mg		bovine	mitochondria	NADH:O2	Positive	IC50	96
Rolliniastatin-1	0.03 nmol/mg		bovine	mitochondria	NADH–Q	Positive	IC50	98
rolliniastatin-2	0.06 nmol/mg		bovine	mitochondria	NADH–Q-1	Positive	IC50	98

T001001. NADH-ubiquinone oxidoreductase chain 1
T001002. NADH-ubiquinone oxidoreductase chain 2
T001003. NADH-ubiquinone oxidoreductase chain 3
T001004. NADH-ubiquinone oxidoreductase chain 4
T001005. NADH-ubiquinone oxidoreductase chain 4L
T001006. NADH-ubiquinone oxidoreductase chain 5
T001007. NADH-ubiquinone oxidoreductase chain 6
T001008. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
T001009. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
T001010. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
T001011. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
T001012. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
T001013. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
T001014. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
T001015. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13
T001016. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
T001017. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
T001018. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
T001019. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
T001020. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
T001021. NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
T001022. NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
T001023. NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
T001024. NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial
T001025. NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
T001026. NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
T001027. NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
T001028. NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
T001029. NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
T001030. Complex I intermediate-associated protein 30, mitochondrial
T001031. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2
T001032. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3
T001033. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
T001034. Arginine-hydroxylase NDUFAF5, mitochondrial
T001035. NADH dehydrogenase (ubiquinone) complex I, assembly factor 6
T001036. Protein arginine methyltransferase NDUFAF7, mitochondrial
T001037. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 8
T001038. Complex I assembly factor ACAD9, mitochondrial
T001039. Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
T001040. FAD-dependent oxidoreductase domain-containing protein 1
T001041. Iron-sulfur protein NUBPL
T001042. Complex I assembly factor TIMMDC1, mitochondrial
T001043. Complex I assembly factor TMEM126B, mitochondrial
T001044. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6
T001045. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
T001046. NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
T001047. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
T001048. NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
T001049. NADH dehydrogenase [ubiquinone] 1 subunit C2
T001050. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
T001051. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
T001052. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
T001053. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial
T001054. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
T001055. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
T001056. NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
T001057. NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
T001058. NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
T001059. Acyl carrier protein, mitochondrial
T001060. quinone
T001061. semiquinone
T001062. quinol
T001063. flavin mononucleotide
T001064. iron-sulfur clusters

F0201. respiration
F02010202. oxygen consumption rate (OCR)
F020103. electron transport chain
F020403. glucose galactose IC50 ratio
F0504. ROS production