Compound	Dose	Time	Species	Model	Method	Action	Result	Positive criterion	Reference
Perhexiline	100 μM	5 minutes preincubation	mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	193
Amiodarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Perhexiline	200 μM	5 minutes preincubation	mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	193
Perhexiline	400 μM	5 minutes preincubation	mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	193
Perhexiline	100 μM	5 minutes preincubation	mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.05	193
benzarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
benzbromarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
benzarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
Pioglitazone						decrease	Positive		22
Gentamicin	5mM	10min	rat	isolated rat liver (and kidney) mitochondria	Oroboros O2K Oxygraph, complex II-based state 4 respiration	increase	Positive		316
promethazine						increase	Positive		22
Gentamicin	5mM	10min	rat	isolated rat liver mitochondria	Oroboros O2K Oxygraph, complex I-based state 4 respiration	increase	Positive		316
benzbromarone	100 μmol/L		rat; Sprague–Dawley	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	4
clomipramine						decrease	Positive		22
Chlorpromazine						increase	Positive		22
Chlorpromazine						decrease	Positive		22
imipramine						increase	Positive		22
Flufenamic Acid	2.9 ± 0.3		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Tolfenamic Acid	3.4 ± 0.6		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Mefenamic acid	17.9 ± 2.2		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Meloxicam	22.8 ± 2.8		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Sudoxicam	37.4 ± 3.0		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Piroxicam	69.0 ± 7.2		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Diflunisal	6.7 ± 1.3		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Diclofenac	56.3 ± 4.9		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Sulindac	> 100		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Sulindac sulfide	25.7 ± 4.5		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Nimesulide	2.6 ± 0.2		rat	isolated rat liver mitochondria	State 2 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	UC50 (nmol/mg mitochondrial protein)	40
Sulindac sulfide	39.8 ± 2.9		rat	isolated rat liver mitochondria	State 3 respiration ( 96-well plate format using a phosphorescent oxygen-sensitive probe MitoXpress)	inhibit	Positive	IC50 (nmol/mg mitochondrial protein)	40
Miconazole	I50=100, 160, 160, 170, 150 μM for substrates Glutamate/Malate, Pyruvate/Malate, Ornithine/Malate, Arginine/Malate and Succinate repectively		rat	liver mitochondria	Clark electrode	inhibit	Positive		303
clomipramine						increase	Positive		22
Nimesulide							Positive		197
Gentamicin	5mM	10min	rat	isolated rat liver (and kidney) mitochondria	Oroboros O2K Oxygraph, complex II-based state 3u respiration	decrease	Positive		316
Gentamicin	5mM	10min	rat	isolated rat liver mitochondria	Oroboros O2K Oxygraph, complex I-based state 3u respiration	decrease	Positive		316
Perhexiline	200 μM		mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	increase	Positive	p < 0.01	193
Metformin	2mM	30 minutes	mouse; C57BL/6J	isolated skeletal muscle mitochondria	Measurement of Oxygen level; incubated in complex I substrate; added ADP (500 μM)	decrease	Positive	p < 0.05	172
Metformin	2mM	30 minutes	mouse; C57BL/6J	isolated skeletal muscle mitochondria	Measurement of Oxygen level; incubated in complex I substrate; added oligomycin (2.5 μg oligomycin/mg mitochondrial protein)	decrease	Positive	p < 0.05	172
Nitrofurantoin	1 mM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake; used glutamate as substrate	decrease	Positive	p < 0.05; 61% inhibition	13
Nitrofurantoin	50 μM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake; used glutamate as substrate	decrease	Positive	p < 0.05; 72% inhibition	13
Nitrofurantoin	5 μM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake; used glutamate as substrate	decrease	Positive	p < 0.05; 38% inhibition	13
Nitrofurantoin	1 mM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake; used β-hydroxybutyrate as substrate	decrease	Positive	p < 0.05	13
Nitrofurantoin	50 μM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake; used β-hydroxybutyrate as substrate	decrease	Positive	p < 0.05; 60% inhibition	13
Nitrofurantoin	5 μM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake; used β-hydroxybutyrate as substrate	decrease	Positive	p < 0.05	13
Nitrofurantoin	1 mM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake; used α-ketoghttarate as substrate	decrease	Positive	p < 0.05	13
Nitrofurantoin	50 μM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake; used α-ketoghttarate as substrate	decrease	Positive	p < 0.05; 70% inhibition	13
Nitrofurantoin	5 μM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake; used α-ketoghttarate as substrate	decrease	Positive	p < 0.05	13
nitrofurazone	1mM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.05; 64% inhibition	13
nitrofurazone	1mM		mouse; C57B/6J	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.05; 63% inhibition	13
Perhexiline	200 μM	5 minutes preincubation	mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	193
Perhexiline	400 μM	5 minutes preincubation	mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	decrease	Positive	p < 0.01	193
nitrofurazone	5μM		mouse; C57B/6J	mitoplasts	Measurement of oxygen uptake	decrease	Positive	p < 0.05	13
nitrofurazone	5μM		mouse; C57B/6J	mitoplasts	Measurement of oxygen uptake	decrease	Positive	p < 0.05	13
Diazepam	70 μg/ml			pig kidney embryo cells	polarographic cell (1.2 ml)	inhibit	Positive		293
buformin	100 nmol/mg mitochondrial protein		rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
Phenformin	100 nmol/mg mitochondrial protein		rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
Metformin	500 nmol/mg mitochondrial protein	40 minutes preincubation	rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
buformin	100 nmol/mg mitochondrial protein	40 minutes preincubation	rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
Phenformin	100 nmol/mg mitochondrial protein	40 minutes preincubation	rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
buformin	100 nmol/mg mitochondrial protein		rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
Phenformin	100 nmol/mg mitochondrial protein		rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
Metformin	500 nmol/mg mitochondrial protein	40 min preincubation	rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
buformin	100 nmol/mg mitochondrial protein	40 min preincubation	rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
Phenformin	100 nmol/mg mitochondrial protein	40 min preincubation	rat; Sprague–Dawley	liver mitochondria	Meassurement of respiration	decrease	Positive		15
Perhexiline	100 μM		mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	increase	Positive	p < 0.01	193
Ketoconazole	I50=32, 74, 65, 75, 500μM for substrates Glutamate/Malate, Pyruvate/Malate, Ornithine/Malate, Arginine/Malate and Succinate repectively		rat	liver mitochondria	Clark electrode	inhibit	Positive		303
Perhexiline	100 μM		mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	increase	Positive	p < 0.01	193
Perhexiline	200 μM		mouse; Crl/CD-l(1CR)BR Swiss	liver mitochondria	Measurement of oxygen uptake	increase	Positive	p < 0.01	193

F02010101. state 1 respiration
F02010102. state 2 respiration
F02010103. state 3 respiration
F02010104. state 4 respiration
F02010105. state 5 respiration
F02010106. state 3u respiration