Compound	Dose	Time	Species	Model	Method	Action	Result	Positive criterion	Reference
MCU-i11	10 μM			Skeletal Muscle Fibers	measured mitochondrial Ca2+ uptake in flexor digitorum brevis (FDB) myofibers freshly isolated from adult mice and previously transfected in vivo with the mitochondria-targeted Ca2+ probe 4mt-GCaMP6f	decrease	Positive		301
DS16570511	7 μM			HEK293A cells	HEK293A cell lines expressing the mitochondria-targeted Ca2+ indicator protein aequorin	decrease	Positive	IC50	302
SB202190	10 μM			intact and in permeabilized HeLa cells (MM5 cells expressing mitmutAEQ )	mitochondrial Ca2+ uptake	increase	Positive		233
kaempferol	1μM				The HeLa cell clone MM5 expressing mitochondrially targeted mutated aequorin was used for [Ca2+]M measurements. [Ca2+]c measurements were carried out using HeLa cells transiently transfected with the plasmid for cytosolic aequorin.	increase	Positive		234
kaempferol	7μM				The HeLa cell clone MM5 expressing mitochondrially targeted mutated aequorin was used for [Ca2+]M measurements. [Ca2+]c measurements were carried out using HeLa cells transiently transfected with the plasmid for cytosolic aequorin.	increase	Positive	EC50	234
quercetin	21 μM				mitochondrial Ca2+ uptake	increase	Positive	EC50	234
genistein	37μM				mitochondrial Ca2+ uptake	increase	Positive	EC50	234
CGP37157	1 μmol/L			periodically paced rat ventricular myocytes (VMs) under β-adrenergic stimulation	mitochondrial Ca2+ monitored by mitochondrial-targeted Ca2+ biosensor mtRCamp1h.		Positive		241
kaempferol	10 μmol/L			periodically paced rat ventricular myocytes (VMs) under β-adrenergic stimulation	mitochondrial Ca2+ monitored by mitochondrial-targeted Ca2+ biosensor mtRCamp1h.		Positive		241
Troglitazone	50 μM		male CD-1 mice	isolated liver mitochondria	Assessment of mitochondrial Ca2+ efflux with arsenazo III at 675/685 nm.	decrease	Positive		331
CGP37157	1 μmol/L						Positive		241
Ru360	2 μmol/L						Positive		241
Ciglitazone	50 μM		male CD-1 mice	isolated liver mitochondria	Assessment of mitochondrial Ca2+ efflux with arsenazo III at 675/685 nm.	decrease	Positive		331
N-ethylmaleimide	20 nmoles/mg protein		bovine	heart mitochondria and submitochondrial particles.	Determination of the energized accumulation of Pi and the binding of ADP; Assay of energized translocation of calcium and Pi	affect	Positive		228
neomycin	400 μM		zebrafish	zebrafish lateral line hair	measrueing cytoplasmic oxidation (HyPer) and mitochondrial Ca2+ uptake (mitoRGECO)	increase	Positive		315
Perhexiline						decrease	Positive		16
Perhexiline						decrease	Positive		16
Diazoxide	EC50= 40mmol/L, saturation by 100mmol/L			Primary Culture of Neonatal Rat Cardiac Ventricular Myocytes	flow cytometry and quantitative image analysis of cells stained with fluorescent DC indicators.	affect	Positive		188
Pinacidil						affect	Positive		188
5-Hydroxydecanoate	500mmol/L			Primary Culture of Neonatal Rat Cardiac Ventricular Myocytes	flow cytometry and quantitative image analysis of cells stained with fluorescent DC indicators.	affect	Positive		188
Glibenclamide						affect	Positive		188
Glibenclamide	K1/2 values 1-6 microM			heart and liver mitochondria from rat		affect	Positive		190
promethazine						increase	Positive		22
clomipramine						increase	Positive		22
imipramine						increase	Positive		22
Chlorpromazine						increase	Positive		22
2,4-dinitrophenol						increase	Positive		24
2,4-dinitrophenol						increase	Positive		24
Trimetazidine	10-100µM		rat	isolated cardiac mitochondria		increase	Positive		274
Diclofenac	50 μmol/L	2 minutes	rat	liver mitochondria	Assessment of Mitochondrial Ca2+ Efflux; Energized with succinate	decrease	Positive	not mentioned	6
MCU-i4	5 μM			HeLa cells transfected with mitochondria-targeted aequorin (mtAeq) after cellular stimulation with histamine	Highigh-throughput screening assay based on aequorin.	decrease	Positive		301
MCU-i11	5 μM			HeLa cells transfected with mitochondria-targeted aequorin (mtAeq) after cellular stimulation with histamine	Highigh-throughput screening assay based on aequorin.	decrease	Positive		301
MCU-i4	10 μM			mouse embryonic fibroblasts (MEFs), MDA-MB-231, and HEK293T cell lines	Small-Scale Validation Assay (cytoplasmic- or mitochondria-targeted aequorin (cytAeq and mtAeq, respectively)	decrease	Positive		301
MCU-i11	10 μM			mouse embryonic fibroblasts (MEFs), MDA-MB-231, and HEK293T cell lines	Small-Scale Validation Assay (cytoplasmic- or mitochondria-targeted aequorin (cytAeq and mtAeq, respectively)	decrease	Positive		301
MCU-i4	8.4 μM(Micu1), 18 μM (Micu1-Micu2)			Purified recombinant Micu1, Micu2	Purified recombinant Micu1, Micu2, and the dimeric Micu1-Micu2 proteins were immobilized on different channels of a SPR sensor chip surface.	decrease	Positive	IC50	301
MCU-i11	2.9 μM(Micu1), 2.7 μM (Micu1-Micu2)			Purified recombinant Micu1, Micu2	Purified recombinant Micu1, Micu2, and the dimeric Micu1-Micu2 proteins were immobilized on different channels of a SPR sensor chip surface.	decrease	Positive	IC50	301
MCU-i4	10 μM			Skeletal Muscle Fibers	measured mitochondrial Ca2+ uptake in flexor digitorum brevis (FDB) myofibers freshly isolated from adult mice and previously transfected in vivo with the mitochondria-targeted Ca2+ probe 4mt-GCaMP6f	decrease	Positive		301

F02110301. transport of calcium
F02110302. accumulation of calcium
F02110303. transport of sodium
F02110304. transport of potassium
F02110305. potassium release
F02110306. accumulation of potassium
F02110307. transport of proton
F02110308. transport of phosphate
F02110309. accumulation of phosphate
F02110310. transport of chlorine
F02110311. transport of anion
F02110312. bicarbonate transport

T205. mCU (mitochondrial calcium uniporter)
T206. mitochondrial Na/Ca2+ exchanger
T208. mitochondrial KATP chanel (mtKATP)
T211. Potassium voltage-gated channel subfamily A member 5
T212. hERG
T213. potassium
T214. Phosphate carrier protein, mitochondrial
T432. Calcium uptake protein 1, mitochondrial