F0212 | Metabolism of cofactors and vitamins

R-HSA-196854
Function of mitochondria >> metabolism of cofactors and vitamins

Compound Dose Time Species Model Method Action Result Positive criterion Reference
Melittin 5μM and 16μM bovine bovine heart MF1-ATPase Positive IC50 170
Nimesulide 1 mM rat; Sprague-Dawley Crl:CD (SD) BR lung microsomes measurement of NADPH oxidation increase Positive p < 0.05 11
IF1 0.25 μM bovine bovine heart MF1-ATPase Positive IC50 170
IF1 1.2 μM bovine heart MF1-ATPase Positive IC50 170
IF1 0.84 μM bovine heart MF1-ATPase Positive IC50 170
IF1 300 μg/mg protein (T. pyriformis SMP-ATPase) ; Positive IC50 170
IF1 34 μg/mg protein C. asciculate SMP-ATPase Positive IC50 170
IF1 0.24 μM rat liver MF1-ATPase Positive Ki 170
Enterostatin Positive 170
ethyldimethylaminopropyl carbodiimide 13 mol of EDC/mol F1 (EF1-ATPase) Positive 95% inhibition 170
1,5-difluoro-2,4-dinitrobenzene Positive 170
Angiostatin Positive 170
NBD-Cl Positive 170
NBD-Cl Positive 170
Gentamicin 300 μg/ml 1hr postnatal day 6 (P6±1d) FVB mice basal turn outer hair cells ( low-frequency processing OHCs) from intact cochlear (organ of Corti) explants scanning confocal microscope (NADH autofluorescence); T1 imaging buffer contained 5 mM glucose. T2 imaging buffer contained 10 mM glucose, 3 mM glutamate and 2 mM pyruvate. decrease Positive 312
Troglitazone >25 µM 1 and 2 hr HepG2 cells Cellular ATP concentrations were measured with a ATP Bioluminescent Somatic Cell Assay Kit decrease Positive 332
Amitriptyline Positive 197
Desipramine Positive 197
lumiracoxib Positive 197
Berberine 25 μM 48, 72 hr K1735-M2 mouse melanoma cells An acid extraction procedure was used to evaluate the intracellular concentration of adenine nucleotides. Adenine nucleotides were separated by reverse-phase high-performance liquid chromatography. Quantification was achieved by employing ATP, ADP, and AMP standard curves. decrease Positive p< 0.05 vs. control for each time of incubation 327
Berberine 10 μM 24 hr K1735-M2 mouse melanoma cells An acid extraction procedure was used to evaluate the intracellular concentration of adenine nucleotides. Adenine nucleotides were separated by reverse-phase high-performance liquid chromatography. Quantification was achieved by employing ATP, ADP, and AMP standard curves. decrease Positive p< 0.05 vs. control for each time of incubation 327
buformin 62.5 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive p < 0.001 15
buformin 62.5 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive p < 0.001 15
Phenformin 62.5 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive p < 0.001 15
Phenformin 62.5 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive p < 0.001 15
Metformin 657 μM 24 hours human hepatocytes Assay of Cellular ATP Content decrease Positive IC50 15
buformin 26.1 μM 24 hours human hepatocytes Assay of Cellular ATP Content decrease Positive IC50 15
Phenformin 11.7 μM 24 hours human hepatocytes Assay of Cellular ATP Content decrease Positive IC50 15
Metformin 1430 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive IC50 15
buformin 43.7 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive IC50 15
Phenformin 12.9 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive IC50 15
Metformin > 2000 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive IC50 15
buformin > 500 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive IC50 15
Phenformin > 500 μM 24 hours human HepG2 cells Assay of Cellular ATP Content decrease Positive IC50 15
Diazepam 150μg/ml 2hr PE cells luciferin-luciferase method decrease Positive 293
Troglitazone human hepatocytes ATP bioluminescence assay decrease Positive 226
Diclofenac 50 μmol/L 10 minutes rat liver mitochondria Measurement of Mitochondrial NADPH; incubated in the presence of Ca2+ increase Positive p < 0.01 6
Diclofenac 250 μmol/L 24 hours rat hepatocytes Assay of Cellular ATP contents; Assay of LDH leakage decrease Positive p < 0.01 6
Diclofenac 500 μmol/L 24 hours rat hepatocytes Assay of Cellular ATP contents; Assay of LDH leakage decrease Positive p < 0.01 6