Compound	Dose	Time	Species	Model	Method	Action	Result	Positive criterion	Reference
Zidovudine						Increase	Positive		307
Acyclovir						Increase	Positive		307
Ganciclovir						Increase	Positive		307
Zalcitabine						Increase	Positive		307
amphotericin B	2.5mg/ml		Aspergillus fumigatus		oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t	induce	Positive		304
Terbinafine	4mg/ml		Aspergillus fumigatus		oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t	induce	Positive		304
itraconazole	4mg/ml		Aspergillus fumigatus		oxidant-sensing fluorescent probe 2′,7′-dichlorofluorescin diacetate t	induce	Positive		304
tacrolimus	50 µg/ml	12hr		INS-1 cells	MitoSOX Red staining to detect mitochondrial superoxide anion (O2−) levels by flow cytometric analysis and e time-lapse images of cells treated for 12 h with MitoSOX Red.	induce	Positive		322
P110	1 µM	2hr		SH-SY5Y cells were treated with P110 (1 µM) followed by incubation of MPP+ (2 mM) for 2 hours.	Mitochondrial superoxide production was determined using the mitochondrial superoxide indicator MitoSOX Red. Total ROS production was determined in SH-SY5Y cells using CM2-HDCFA.	reduce	Positive		310
neomycin	50 μM		zebrafish	zebrafish lateral line hair	use ROS indicator dye cellROX green ( it labels a number of intracellular compartments, including cytoplasm, nucleus, and mitochondria)	increase	Positive		315
Diflunisal	558		rat	hepatocytes	use CM-H2DCFDA to monitor reactive oxygen species		Positive	AC50 (μM)	40
Sulindac sulfide	109		rat	hepatocytes	use CM-H2DCFDA to monitor reactive oxygen species		Positive	AC50 (μM)	40
Nimesulide	574		rat	hepatocytes	use CM-H2DCFDA to monitor reactive oxygen species		Positive	AC50 (μM)	40
Staurosporine	2 µM	24hr		U2OS	mt-roGFP and mitoSOX	increase	Positive		313
camptothecin	30 µM	24hr		U2OS	mt-roGFP and mitoSOX	increase	Positive		313
Menadione	20 µM	24hr		U2OS	mt-roGFP and mitoSOX	increase	Positive		313
Cisplatin	150 µM	24hr		U2OS	U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria	increase	Positive		313
camptothecin	30 µM	24hr		U2OS	U2OS cells stably expressing mt-roGFP and HyPer-red targeted at mitochondria	increase	Positive		313
Ciprofloxacin						Increase	Positive		307
Mitoquinone				U2OS	mt-roGFP ratio change (Quantitative representation of the percentage of cell death in cells pre-treated with MitoQ followed by drug treatment or drug alone as indicated for 24 h)	reduce	Positive		313
Cisplatin						Increase	Positive		307
Cyclophosphamide						Increase	Positive		307
Gentamicin	5mM	10min	rat	isolated rat liver (and kidney) mitochondria	Mitochondrial ROS production can be measured using Amplex Red, a dye that fluoresces dependent on hydrogen peroxide (H2O2) levels.	reduce	Positive		316
glyceraldehyde 3-phosphate	2.5mM					decrease	Positive		162
dihydroxyacetone phosphate						decrease	Positive		162
MG-132	20 μM	24 hr	rat	PC12 cells	Measurement of intracellular ROS formation with DCFH2-DA	increase	Positive		289
Amitriptyline							Positive		197
3-morpholinosydnonimine	150 μM	24 hr	rat	PC12 cells	Measurement of intracellular ROS formation with DCFH2-DA	decrease	Positive		289
Desipramine							Positive		197
Valproic Acid						increase	Positive		197
Doxorubicin						increase	Positive		197
Efavirenz						increase	Positive		197
Propofol						increase	Positive		197
Ketamine						increase	Positive		197
Manganese	15 mg/l in drinking water		Swiss Albino mice			increase	Positive		242
Paraquat	10 mg/kg/week intraperitoneal (i.p.)	4 weeks	C57BL/6 mice				Positive		242
Paraquat	250 μM	5min	Sprague Dawley rat	brain cultures			Positive		242
Rotenone	0.1–0.5 nmol/mg protein		Wistar rat	brain isolated mitochondria		increase	Positive		242
Cisplatin	84.96 ± 3, 158.9 ± 8.2, 372.7 ± 17.5 and 499.5 ± 15.1 μM	24 hr		Ishikawa, MDA-MB-231, PC-3 and Caco-2	Intracellular ROS (a) and mitochondrial superoxide were measured by fluorescence intensity of H2DCFDA and MitoSOX respectively.	increase	Positive	IC50	299
Dequalinium Chloride	14.24 ± 0.59, 57.85 ± 3.59, 93.31 ± 3.21 and 179.2 ± 5.2 μM	24 hr		Ishikawa, MDA-MB-231, PC-3 and Caco-2	Intracellular ROS (a) and mitochondrial superoxide were measured by fluorescence intensity of H2DCFDA and MitoSOX respectively.	increase	Positive	IC50	299
Amiodarone	10 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Chlorpromazine	50 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Aluminium	10 mg/kg/day intragastric for 12 weeks		Wistar rats				Positive		242
Cyclosporin A	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
imipramine	50 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Maprotiline	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Rotenone	100 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Tamoxifen	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Troglitazone	10 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
atorvastatin	100 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Cerivastatin	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
fluvastatin	50 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
lovastatin	10 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
pravastatin	50 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Simvastatin	5 µM	1 hour	Human	HepG2	High-content screening assay	Increase	Positive	MEC	306
Methylmercury	15 mg/l in drinking water		Swiss albino mice				Positive		242

F050401. H2O2 production

T001. NADH:ubiquinone reductase
T004. Quinol--cytochrome-c reductase
T181. Glycerol-3-phosphate dehydrogenase, mitochondrial
T358. Dynamin-1-like protein
T387. Reactive oxygen species